Table 5 Concentrations of cytokines, cytokine related factors and chemokines (median, IQR, pg/mL) in pre-patients stratified for the presence or absence of IgA and IgG isotypes of anti-CCP2 antibodies
IgA positive | IgA negative | P-value | IgG positive | IgG negative | P-value | |
|---|---|---|---|---|---|---|
(n= 14) | (n= 38) | (n= 20) | (n= 32) | |||
Cytokine/chemokine | ||||||
General activation | ||||||
IL-1β | 7.2 (2.9 to 36.4) | 3.4 (2.3 to 5.1) | 0.025 | 5.9 (2.3 to 25.5) | 3.3 (2.3 to 4.9) | 0.071 |
IL-1Ra | 164.6 (128.7 to 454.9) | 144.1 (96.5 to 195.5) | 0.089 | 162.9 (109.7 to 408.6) | 144.1 (99.8 to 186.5) | 0.136 |
IL-2Rα | 66.9 (38.6 to 110.0) | 33.4 (22.3 to 51.3) | 0.008 | 60.9 (35.9 to 88.7) | 32.2 (20.8 to 47.6) | <0.001 |
TNFα | 108.1 (3.0 to 506.7) | 49.1 (25.7 to 118.6) | 0.556 | 85.4 (27.2 to 224.0) | 45.0 (20.8 to 112.9) | 0.234 |
IL-6 | 19.8 (5.9 to 147.7) | 4.3 (1.1 to 11.5) | 0.009 | 16.2 (3.6 to 111.6) | 4.8 (1.1 to 8.2) | 0.019 |
IL-2 | 74.7 (2.8 to 141.5) | 3.8 (1.1 to 18.3) | 0.020 | 49.1 (10.5 to 106.8) | 1.4 (1.1 to 11.7) | <0.001 |
IL-15 | 2.4 (0.2 to 9.6) | 0.5 (0.2 to 4.2) | 0.355 | 4.2 (0.8 to 4.2) | 0.5 (0.2 to 4.2) | 0.010 |
Th1 related | ||||||
IL-12 | 54.4 (21.0 to 135.2) | 21.2 (13.7 to 32.3) | 0.033 | 53.5 (21.4 to 161.3) | 20.5 (13.5 to 29.3) | 0.012 |
IFN-γ | 113.4 (84.5 to 1711.0) | 92.9 (50.5 to 150.1) | 0.083 | 152.2 (97.1 to 1167.6) | 78.5 (50.4 to 130.8) | 0.022 |
Th2 related | ||||||
IL-4 | 4.4 (3.2 to 26.4) | 3.0 (2.2 to 4.2) | 0.044 | 4.4 (3.3 to 17.4) | 2.9 (2.3 to 4.0) | 0.019 |
IL-5 | 5.6 (1.3 to 12.0) | 5.8 (3.3 to 7.7) | 0.675 | 5.8 (1.3 to 12.0) | 5.8 (3.6 to 7.3) | 0.441 |
IL-9 | 128.3 (22.7 to 283.6) | 19.6 (5.7 to 85.9) | 0.022 | 121.1 (24.7 to 255.9) | 17.6 (5.6 to 71.7) | 0.012 |
IL-13 | 5.1 (3.6 to 11.2) | 4.4 (2.9 to 6.7) | 0.329 | 5.3 (3.9 to 17.0) | 4.0 (3.0 to 6.5) | 0.089 |
Eotaxin | 80.7 (43.2 to 429.8) | 42.0 (26.1 to 53.1) | 0.008 | 80.7 (44.0 to 363.8) | 36.7 (26.0 to 47.6) | 0.004 |
Th17 related | ||||||
IL-17 | 31.3 (17.7 to 46.5) | 22.3 (9.9 to 38.1) | 0.082 | 31.5 (20.3 to 42.0) | 21.2 (9.6 to 36.9) | 0.040 |
Treg cell-related | ||||||
IL-10 | 6.5 (3.8 to 19.8) | 5.5 (3.6 to 8.1) | 0.279 | 6.2 (3.2 to 18.4) | 5.3 (3.9 to 7.6) | 0.541 |
Bone marrow derived | ||||||
IL-7 | 23.8 (19.2 to 36.8) | 27.2 (18.6 to 37.4) | 0.807 | 24.8 (13.4 to 42.1) | 26.2 (20.8 to 36.6) | 0.620 |
GM-CSF | 23.0 (12.9 to 103.4) | 5.0 (2.5 to 23.9) | 0.008 | 20.2 (5.5 to 62.0) | 4.6 (2.5 to 15.7) | 0.054 |
G-CSF | 57.1 (39.0 to 80.1) | 58.7 (42.3 to 74.5) | 0.463 | 57.1 (38.8 to 83.1) | 58.7 (43.6 to 72.7) | 0.527 |
Stromal cells and angiogenic factors | ||||||
bFGF | 6.7 (2.2 to 14.1) | 6.6 (2.2 to 6.8) | 0.187 | 2.9 (1.6 to 11.2) | 6.6 (3.5 to 6.8) | 0.519 |
PDGF-BB | 1905.9 (705.9 to 2387.4) | 1985.6 (997.6 to 3160.0) | 0.911 | 1975.9 (1109.6 to 3437.3) | 1695.3 (950.6 to 2370.9) | 0.312 |
VEGF | 24.6 (10.0 to 63.7) | 13.1 (5.7 to 23.9) | 0.029 | 28.6 (12.0 to 53.1) | 11.7 (4.9 to 15.0) | 0.022 |
Chemokines | ||||||
MIF | 367.1 (90.8 to 538.2) | 223.0 (142.1 to 461.5) | 0.919 | 384.5 (163.2 to 599.4) | 207.8 (123.7 to 366.5) | 0.095 |
MIG | 488.5 (319.0 to 1167.5) | 272.9 (190.4 to 371.2) | 0.004 | 425.6 (279.1 to 741.3) | 272.9 (201.6 to 364.0) | 0.039 |
IL-8 | 7.7 (3.6 to 11.4) | 5.0 (0.5 to 10.2) | 0.263 | 6.4 (0.5 to 12.1) | 6.6 (1.7 to 9.4) | 0.975 |
IP-10 | 962.1 (696.2 to 2214.2) | 634.3 8425.0 to 1062.0) | 0.014 | 957.0 (644.2 to 1855.8) | 624.4 (420.0 to 1071.5) | 0.061 |
MCP-1 | 23.0 (19.0 to 62.4) | 18.5 (12.8 to 28.4) | 0.028 | 25.8 (17.6 to 52.7) | 17.3 (12.9 to 26.3) | 0.085 |
MIP-1α | 7.2 (4.2 to 11.7) | 6.9 (4.8 to 10.0) | 0.746 | 7.4 (2.9 to 11.9) | 6.9 (5.1 to 9.3) | 0.994 |
MIP-1β | 44.2 (37.6 to 49.7) | 35.7 (26.5 to 42.2) | 0.030 | 42.9 (31.4 to 48.7) | 36.5 (27.4 to 41.1) | 0.200 |