Figure 4

Glucosamine up-regulated TNF-αR1 and down-regulated IFN-γR1 expression in CIOA on synovial CD3 T cells. Synovial cells were obtained at Day 30 from joint extracts of healthy (n = 5) mice, CIOA mice treated with PBS (CIOA; n = 5) and CIOA mice treated with glucosamine (CIOA + Glu1, n = 5). Synovial cells were stained with PE-labeled antibody against CD3 and with biotinylated antibodies against TNF-αR1 and IFN-γR1 followed by avidin-FITC staining and were subjected to flow cytometry. Data represent the mean of fluorescence expression ± SD from two independent experiments involving five mice/group; Student's t-test; ***P < 0.001.