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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells

Figure 2

Quantification of proteoglycan in MSC pellets. Di-methyl methylene Blue (DMMB) analysis was used to assess the amount of Glycosaminoglycan (GAG) associated with the human mesenchymal stem cell (MSC) pellet 21 days post culture with four media groups; B = Basal, C = Chondrogenic, NCA = notochordal NP cells in alginate; NCT = notochordal NP cells in tissue normalized to DNA content using the Picogreen assay (μgGAG/μgDNA). Bar indicates significance, P < 0.05. Significantly more GAG was associated with the pellets of the NCT group in comparison to all other media groups.

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Arthritis Research & Therapy

ISSN: 1478-6362

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