Table 2 Fold changes in gene expression data for media conditions B, C, NCA and NCT Significance greater than two-fold relative to Day 0 MSCs or Basal in bold and underlined; + = fold increase & - = fold decrease (P < 0
ALL GENES | Normalized to Day 0 | Normalized to Basal | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | B | C | NCA | NCT | C | NCA | NCT |
| Â | Â | BGN | -1.0 | +1.1 | -6.1 | +1.6 | +1.1 | -6.2 | +1.6 |
| Â | Â | GPC1 | +2.5 | +2.1 | +1.5 | +1.9 | -1.2 | -1.7 | -1.2 |
| Â | IVD | KRT19 | -7.3 | -1.0 | -1.4 | -12.7 | -1.0 | +1.1 | -1.6 |
Phenotypic | Â | LAMB1 | +1.6 | +1.3 | -1.2 | +1.3 | -1.3 | -2.0 | -1.2 |
| Â | Â | SOX9 | +2.59 | +1.48 | -1.32 | +6.79 | -1.65 | -3.49 | +2.74 |
| Â | MSC | BGLAP | +2.02 | -1.13 | +1.95 | +3.51 | -2.37 | -1.12 | +1.58 |
| Â | Â | PPARG | +27.62 | +9.90 | +9.53 | +19.86 | -2.79 | +1.24 | -1.41 |
| Â | Â | ACAN | -14.1 | -6.3 | -2.5 | -16.2 | +2.1 | -1.2 | -1.2 |
| Â | Â | COL1A1 | -1.1 | +1.2 | -29.7 | -3.4 | +1.3 | -27.3 | -2.9 |
| Â | Â | COL10A1 | +1.1 | +60.4 | +2.2 | +61.9 | +52.1 | +1.7 | +51.8 |
Matrix proteins | COL2A1 | -1.0 | +49.2 | -1.0 | +15.4 | +32.2 | -1.0 | +8.1 | |
| Â | Â | COL3A1 | +3.6 | +3.9 | -11.7 | -2.2 | +1.1 | -42.9 | -7.4 |
| Â | Â | eln | -1.4 | +12.9 | -3.0 | -2.4 | +17.4 | -20.8 | -1.8 |
| Â | Â | HAS1 | -7.3 | +1.3 | +1.0 | +1.1 | 11.4 | +7.5 | +9.3 |
| Â | Aggrecanases | ADAMTS4 | +6.4 | +4.9 | +2.2 | +26.4 | -1.3 | -2.9 | +4.2 |
| Â | Â | ADAMTS5 | +1.1 | -2.5 | +3.2 | +5.1 | -2.6 | +2.9 | +5.2 |
Matrix enzyme | Â | MMP1 | +369.3 | +9.7 | +86039.1 | +28040.4 | -3.2 | +231.6 | +78.2 |
| Â | MMPs | MMP13 | +2637.8 | +3056.0 | +1085.0 | +15582.2 | +1.1 | -2.5 | +6.0 |
| Â | Â | MMP14 | +4.5 | +2.2 | +6.2 | +6.8 | -2.1 | +1.3 | +1.5 |
| Â | Â | MMP2 | +3.8 | +1.8 | +4.7 | +4.0 | -2.1 | +1.2 | +1.0 |
| Â | Â | MMP3 | +36.1 | +184.4 | +203663.7 | +1973.3 | +11.7 | +12436.5 | +120.9 |
| Â | Â | MMP9 | +22.2 | +5.9 | +19.1 | +3.2 | -3.8 | -1.1 | -7.3 |
| Â | Â | TIMP1 | +4.99 | +1.77 | +5.90 | +9.12 | -2.60 | +1.26 | +1.79 |
Anti-catabolic | TIMP2 | +1.46 | -1.10 | +1.26 | +1.66 | -1.64 | -1.09 | +1.14 | |
| Â | Â | TIMP3 | -1.00 | -1.00 | -1.00 | +3.68 | -1.00 | -1.00 | +3.08 |
| Â | Â | TGFB1 | +1.82 | +1.92 | +2.01 | +3.96 | +1.06 | +1.07 | +2.11 |
| Â | Â | TGFB2 | +1.43 | -3.34 | -4.91 | -1.80 | -4.86 | -6.78 | -2.49 |
Growth factors | TGFB | TGFB3 | +8.31 | +1.36 | +3.08 | +5.27 | -6.23 | -2.97 | -1.58 |
| Â | Â | TGFBR1 | -4.52 | -4.61 | -1.03 | +1.05 | -1.00 | +4.19 | +4.83 |
| Â | Â | TGFBR2 | +1.03 | +1.65 | +1.25 | +1.17 | 1.59 | +1.18 | +1.11 |
| Â | Â | CTGF | -8.9 | -3.9 | -76.5 | -9.6 | +2.4 | -8.2 | -1.0 |
| Â | Â | EGF | -3.3 | -7.1 | -1.1 | -1.3 | -2.1 | +1.5 | +2.4 |
| Â | Â | FGF1 | -1.6 | -1.5 | +1.6 | +9.2 | +1.1 | +2.4 | +14.1 |
| Â | General | IGF1 | +169.7 | +104.0 | -1.0 | +87.4 | -1.6 | -1.0 | -1.9 |
| Â | Â | PDGFA | -1.63 | -1.57 | -1.39 | -1.06 | +1.02 | +1.15 | +1.46 |
| Â | Â | WISP1 | +3.27 | +2.62 | -2.44 | +1.90 | -1.35 | -8.23 | -1.75 |
| Â | Â | IL1B | +22.7 | +15.1 | +9911.6 | +444.5 | -2.1 | +310.6 | +14.7 |
| Â | Â | TNF | +1.01 | +1.66 | +90.93 | +5.33 | +1.56 | +72.86 | +3.32 |
Inflammatory | CASP3 | +1.3 | -1.0 | -1.2 | +1.2 | -1.3 | -1.8 | -1.1 | |
| Â | Â | BDNF | -12.9 | -4.7 | -1.3 | -6.3 | -1.2 | +3.1 | +2.0 |
| Â | Â | NGF | -4.0 | -2.7 | +1.4 | -4.4 | -1.6 | +1.6 | -1.1 |
| Â | Â | TAC4 | -1.08 | +1.03 | +4.22 | +1.28 | +1.09 | +4.33 | +1.36 |