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Table 1 Chips and markers used on the IMPACT platform*

From: Novel multiplex technology for diagnostic characterization of rheumatoid arthritis

Chip name

Chip components

 

Antigens

Capture antibodies

Synovial antigen chip 1

Histone 2B/e (1-20)

 
 

Biglycan (247-266)

 
 

Fibromodulin (246-265)

 
 

Vimentin (58-77) (Cit 64, 69, 71)

 
 

Acetyl-calpastatin (184-210)

 
 

Fibromodulin (201-220)

 
 

Profilaggrin (293-310) (Cit 301, 302)

 
 

Clusterin (170-188)

 
 

Fibrinogen A (31-50) (Cit 35, 38, 42)

 
 

Fibrinogen A (616-635) (Cit 621, 627, 630)

 

Synovial antigen chip 2

Histone 2A (95-114)

 
 

Profilaggrin (293-310) (Cit 301, 305)

 
 

HSP60 (287-297)

 
 

Serine protease 11 (433-452)

 
 

Osteoglycin (177-196)

 
 

Apolipoprotein E (277-296) (Cit 278, 292)

 
 

Clusterin (334-353) (Cit 336, 339)

 
 

COMP (453-472)

 

CID 1

 

anti-CRP

  

anti-IgA (for RF measurement)

  

anti-IgM (for RF measurement)

CID 3 chip 1

Cit peptide 1

 
 

Cit peptide 2

 
 

Cit peptide 3

 
 

Cit peptide 4

 

CID 3 chip 2

Cit peptide 5

 
 

Cit peptide 6

 
 

Cit peptide 7

 
 

Cit peptide 8

 
 

Cit peptide 9

 
 

Cit peptide 10

 
 

Cit peptide 11

 

CID 4

 

anti-MMP 3

  

anti-IL-6

  

anti-S100 protein A8/A9

  

anti-E-Selectin

  

anti-HABP

Bone

 

anti-PTH

  

anti-βCrosslaps

  

anti-Osteocalcin

  

anti-P1NP

  1. *Candidate rheumatoid arthritis antigens were spotted on the chip for measurement of autoantibody reactivities. Primary antibodies were spotted on the chip for measurement of analyte (e.g. inflammatory mediators and products of bone turnover) levels.
  2. Cit, citrullinated; HSP 60, heat shock protein 60; COMP, cartilage oligomeric matrix protein; CRP, C-reactive protein; MMP3, matrix metalloproteinase 3; IL-6, interleukin-6; HABP, hyaluronic acid binding protein; PTH, parathyroid hormone; P1NP, procollagen type 1 amino-terminal propeptide.
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Arthritis Research & Therapy

ISSN: 1478-6362

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