Figure 3

Functional analysis of rat FLSs co-cultured with pristane-primed T cells. Rat FLSs were co-cultured with pristane-primed or control T cells, and relative mRNA expression of genes in FLSs was detected by real-time PCR. (A) TLR3 mRNA expression in FLSs was measured 24 hours after co-culture with series FLS:T-cell ratios of 5:1, 1:1, 1:5 and 1:10. (B) FLS migration ability was analyzed 24 and 48 hours after co-culture with FLS:T-cell ratios of 1:1 and 1:5. The migration ability of FLSs co-cultured with pristane-primed T cells was compared to that co-cultured with control T cells. (C) Cytokine (IFN-β and IL-6) and matrix metalloproteinase MMP3 and MMP13 expression were detected 24 hours after co-culture with the cell ratio 1:10. Their expression in FLSs co-cultured with pristane-primed T cells was compared to that co-cultured with control T cells. Data are presented as means ± SEM of four replicated determinations from three independent experiments. Levels of significance were calculated by using Student's t-test (*P < 0.05, ***P < 0.001).