Figure 2

Histopathology images and scores from CIA mice. (a) Histopathology images from collagen-induced arthritis (CIA) mice: 1, 2, 5, and 6 were treated with vehicle; 3, 4, 7, and 8 were treated with 12.5 mg/kg PCI-32765; 1, 2, 3, and 4 were from carpus, 5, 6, 7, and 8 were from tarsus. Images 1, and 3 are magnified 40 ×; 2, 4, 5, 6, 7, and 8 are magnified 100× under the microscope. Bone resorption is depicted with arrowheads; asterisks show carpal bones in panels 1, and 3. R, radius; U, ulna; MC, metacarpal. In panel 5, and 6 asterisks show severe inflammation, and arrows show loss and necrosis of cartilage. In panels 7, and 8 arrowheads show minimum synovial inflammation, and arrow points to minor loss of articular chondrocytes. (b) Histopathology Scores from four joints (carpus and tarsus, both sides) of the CIA mice were averaged and scored by degree of inflammation, pannus, cartilage damage, and bone damage. * P < 0.05, ** P < 0.01; *** P < 0.001 compared with vehicle, analysis of variance.