(A) Day 8 | ||||||||
---|---|---|---|---|---|---|---|---|
Treatment | ||||||||
PBS (n = 4) | W3/25 (n = 2) | OX35 (n = 4) | RIB5/2 (n = 3) | |||||
Antigen (FACS) | % | MFI | % | MFI | % | MFI | % | MFI |
Isotype control | 0 ± 0.0 | 107 ± 9.0 | 4 ± 0.5 | 98 ± 2.0 | 1 ± 0.3 | 82 ± 3.3 | 0 ± 0.0 | 86 ± 7.7 |
CD3 | 70 ± 5.5 | 122 ± 1.7 | 47 ± 5.0 | 99 ± 1.5 | 31 ± 1.9* | 93 ± 1.5* | 28 ± 7.2* | 95 ± 0.7* |
CD8 | 21 ± 1.0 | 162 ± 1.3 | 27 ± 3.0 | 110 ± 1.5 | 33 ± 2.0* | 109 ± 5.0* | 39 ± 3.2* | 111 ± 0.3* |
CD4 (W3/25) | 55 ± 3.2 | 116 ± 0.8 | 7 ± 2.5 | 98 ± 1.5 | 23 ± 1.7* | 97 ± 1.2* | 8 ± 1.5* | 95 ± 0.7* |
CD4 (OX35) | 52 ± 7.5 | 150 ± 0.9 | 45 ± 8.0 | 93 ± 3.0 | 26 ± 4.1 | 122 ± 2.4 | 8 ± 1.2* | 121 ± 1.0* |
Mouse IgG | 1 ± 0.3 | 105 ± 1.9 | 18 ± 2.0 | 101 ± 0.5 | 2 ± 0.3 | 79 ± 2.4* | 1 ± 0.0 | 85 ± 3.2* |
(B) Day 13 | ||||||||
Treatment | ||||||||
PBS (n = 3) | W3/25 (n = 4) | OX35 (n = 4) | RIB5/2 (n = 3) | |||||
Antigen (FACS) | % | MFI | % | MFI | % | MFI | % | MFI |
Isotype control | 1 ± 0.6 | 78 ± 1.3 | 2 ± 1.7 | 87 ± 4.1 | 1 ± 0.3 | 81 ± 3.7 | 1 ± 0.3 | 71 ± 1.7 |
CD3 | 53 ± 2.3 | 142 ± 0.6 | 44 ± 2.3 | 116 ± 3.0* | 39 ± 2.5* | 94 ± 1.0* | 38 ± 1.5* | 96 ± 2.0* |
CD8 | 20 ± 3.8 | 144 ± 1.5 | 23 ± 1.7 | 128 ± 3.2* | 30 ± 7.4 | 108 ± 1.6* | 41 ± 2.3* | 113 ± 0.9* |
CD4 (W3/25) | 46 ± 1.0 | 110 ± 1.9 | 28 ± 2.0* | 109 ± 1.9 | 25 ± 2.9* | 104 ± 1.6 | 20 ± 0.9* | 106 ± 0.7 |
CD4 (OX35) | 46 ± 0.7 | 154 ± 0.7 | 29 ± 3.8* | 144 ± 5.9 | 23 ± 5.3* | 102 ± 2.6* | 17 ± 2.5* | 116 ± 6.4* |
Mouse IgG | 4 ± 2.5 | 77 ± 2.5 | 9 ± 2.0 | 91 ± 3.5* | 1 ± 0.2 | 82 ± 2.7 | 2 ± 0.0 | 76 ± 1.2 |