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Supplementary Table 2 Cytofluorometric analyses of peripheral blood mononuclear cells on day 8 (A) and day 13 (B) of anti-CD4 treatment (data from one representative of three in vivo experiments).

From: Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on NF-κB binding activity and TNF-α secretion of T cells

(A) Day 8

 

Treatment

 

PBS (n = 4)

W3/25 (n = 2)

OX35 (n = 4)

RIB5/2 (n = 3)

Antigen (FACS)

%

MFI

%

MFI

%

MFI

%

MFI

Isotype control

0 ± 0.0

107 ± 9.0

4 ± 0.5

98 ± 2.0

1 ± 0.3

82 ± 3.3

0 ± 0.0

86 ± 7.7

CD3

70 ± 5.5

122 ± 1.7

47 ± 5.0

99 ± 1.5

31 ± 1.9*

93 ± 1.5*

28 ± 7.2*

95 ± 0.7*

CD8

21 ± 1.0

162 ± 1.3

27 ± 3.0

110 ± 1.5

33 ± 2.0*

109 ± 5.0*

39 ± 3.2*

111 ± 0.3*

CD4 (W3/25)

55 ± 3.2

116 ± 0.8

7 ± 2.5

98 ± 1.5

23 ± 1.7*

97 ± 1.2*

8 ± 1.5*

95 ± 0.7*

CD4 (OX35)

52 ± 7.5

150 ± 0.9

45 ± 8.0

93 ± 3.0

26 ± 4.1

122 ± 2.4

8 ± 1.2*

121 ± 1.0*

Mouse IgG

1 ± 0.3

105 ± 1.9

18 ± 2.0

101 ± 0.5

2 ± 0.3

79 ± 2.4*

1 ± 0.0

85 ± 3.2*

(B) Day 13

 

Treatment

 

PBS (n = 3)

W3/25 (n = 4)

OX35 (n = 4)

RIB5/2 (n = 3)

Antigen (FACS)

%

MFI

%

MFI

%

MFI

%

MFI

Isotype control

1 ± 0.6

78 ± 1.3

2 ± 1.7

87 ± 4.1

1 ± 0.3

81 ± 3.7

1 ± 0.3

71 ± 1.7

CD3

53 ± 2.3

142 ± 0.6

44 ± 2.3

116 ± 3.0*

39 ± 2.5*

94 ± 1.0*

38 ± 1.5*

96 ± 2.0*

CD8

20 ± 3.8

144 ± 1.5

23 ± 1.7

128 ± 3.2*

30 ± 7.4

108 ± 1.6*

41 ± 2.3*

113 ± 0.9*

CD4 (W3/25)

46 ± 1.0

110 ± 1.9

28 ± 2.0*

109 ± 1.9

25 ± 2.9*

104 ± 1.6

20 ± 0.9*

106 ± 0.7

CD4 (OX35)

46 ± 0.7

154 ± 0.7

29 ± 3.8*

144 ± 5.9

23 ± 5.3*

102 ± 2.6*

17 ± 2.5*

116 ± 6.4*

Mouse IgG

4 ± 2.5

77 ± 2.5

9 ± 2.0

91 ± 3.5*

1 ± 0.2

82 ± 2.7

2 ± 0.0

76 ± 1.2

  1. Results of surface staining are expressed as the mean ± SEM of the percentage of positive cells and the mean fluorescence intensity (MFI). FACS = flow cytometry. *P ≤ 0.05 in comparison with PBS control.