Supplementary Figure 2From: Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatantQuantitation of viral RNA by slot blot hybridization analysis after concentration of virus by centrifugation at 6000 g. Viral supernatant was centrifuged at 6000 g for the time periods indicated. The viral pellet was resuspended in a thirtieth of the original volume. The indicated volumes of (a) unconcentrated supernatant, (b) the resuspended viral pellet, and (c) the post-centrifugation supernatant were loaded onto a nylon membrane in a 48-well slot blot format, hybridized with an enhanced green fluorescent protein probe, and exposed to film. Experiments were repeated three times with similar results.Back to article page