Supplementary Table 2 PCR conditions
From: Production of interleukin-1 receptor antagonist by human articular chondrocytes
cDNA | Forward primer | Reverse primer | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|---|
icIL-1Ra1 | A | C | 55 | 577 |
sIL-1Ra | B | C | 55 | 521 |
Unspliced IL1-Ra | B | D | 55 | 303 |
β-Actin | E | F | 60 | 579 |
sIL-1Ra (LC) | B | G | 57 | 268 |
GAPDH (LC) | H | I | 57 | 191 |