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Figure 2 | Arthritis Research & Therapy

Figure 2

From: IgG subclass antibodies to human and bacterial HSP60 are not associated with disease activity and progression over time in axial spondyloarthritis

Figure 2

SDS-PAGE gel of bacterial and human HSP60. Size and purity of each recombinant protein were evaluated using 10% SDS-PAGE gel. The different HSP60 proteins for ELISA were purified using a Ni+ column and imidazole as a competing reagent. Vector transformation was confirmed by isopropyl-β-D-thiogalactopyranoside (IPGT) induction. Lane A: Molecular weight marker. Lanes B, E, H and K: Purified HSP60. Lanes C and D, F and G, I and J and L and M: Total protein content from Escherichia coli strain BL21 (DE3) with (+) and without (-) IPGT induction.

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Arthritis Research & Therapy

ISSN: 1478-6362

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