Figure 2

Proapoptotic effects of DPTTS analyzed with fluorescence-activated cells sorting. (a) Normal and HOCl fibroblasts were incubated with 40 μM DPTTS for 5, 10, or 15 hours. The ratio of apoptosis to necrosis was analyzed with flow cytometry by using the Membrane Permeability/Dead Cell Apoptosis Kit with YO-PRO-1 and propidium iodide. Necrotic cells were PI positive, and apoptotic cells were YO-PRO-1 positive. One of three representative experiments is shown. (b) Kinetics of the apoptosis/necrosis ratio of normal and HOCl fibroblasts treated with 40 μM DPTTS.