Figure 4

Effect of Porphyromonas gingivalis on serum responses after collagen-induced arthritis induction. Mice gavaged with P. gingivalis (Pg) in the oral cavity for 15 days (D0 to D15) were immunized with collagen II (CII) emulsified in complete Freund’s adjuvant (CFA) and incomplete Freund’s adjuvant (IFA) 15 days later (D30), as described in Methods. (A) Antibodies to CII were measured by enzyme-linked immunosorbent assay at D44 and at D73. Serum collected at D44 and D73 were evaluated for interleukin (IL)-17A/interferon (IFN)γ and IL-17 F/IFNγ ratios in (B) CFA/CII immunizations and (C) IFA/CII immunizations. Error bars represent ± standard error of the mean of eight mice per group, P <0.05, analysis of variance and Student’s t test. P <0.2 results are also presented.