Figure 2
From: Evidence that TNF-β (lymphotoxin α) can activate the inflammatory environment in human chondrocytes
IL-1β induces TNF-β protein expression in chondrocytes in a time- and dose-dependent manner. Primary human chondrocytes were either treated with 10 ng/ml interleukin 1β (IL-1β) for the indicated time points (A) or treated with various concentrations of IL-1β for 1 hour (B). Whole-cell lysates were prepared and analyzed by Western blotting with antibodies against tumor necrosis factor β (TNF-β). The results shown are representative of three independent experiments. Housekeeping protein β-actin served as a loading control in all experiments.