Figure 3
miR-26a and toll-like receptor (TLR)3 expression during bone marrow-derived macrophage (BMDM) induction. (A) RT-qPCR results of tlr3 mRNA and miR-26a expression and (B) western blotting results of TLR3 protein expression on day (D)0, D3 and D6 during BMDM induction. Bone marrows were obtained from three DA rats. U6 snRNA and glyceraldehyde-3-phosphate dehydrogenase (gapdh) were used as internal controls in RT-qPCR for miRNA and mRNA expression detection, respectively. Bars represent the standard error of the mean from three rats. *Statistically significant differences (Mann–Whitney U-test, P <0.05). One representative plot and quantitative data from three independent western blotting tests are shown. Ratio indicates the optical intensity of TLR3 protein bands against GAPDH.