Table 1 Genes and primers for RT-qPCR and 3′ UTR cloning
Gene symbol (accession number) | Primer name | Annealing temperature (°C) | Sequences (from 5′ to 3′) |
|---|---|---|---|
TLR3 [GenBank:NM_198791] | Sense | 60 | GATTGGCAAGTTATTCGTC |
Antisense | GCGGAGGCTGTTGTAGG | ||
3′UTR sense | 60 | CGAGCTCTTTGGAGTCAGTGAAGGGAT | |
3′UTR antisense | GAAGCTTCCCATGTATTTATTTGGAGCAA | ||
3′UTR mutation sense | 58 | GTCTGAGTTTTTCATCAAAGTTTGTAT | |
3′UTR mutation Antisense | ATACAAACTTTGATGAAAAACTCAGAC | ||
GAPDH [GenBank:NM_017008] | Sense | 65 | CGGCAAGTTCAACGGCACAG |
Antisense | GAAGACGCCAGTAGACTCCACGAC | ||
IFNB [GenBank:NM_019127] | Sense | 54 | CTTGGGTGACATCCACGACTAC |
Antisense | GGCATAGCTGTTGTACTTCTTGTCTT | ||
TNFA [GenBank:NM_012675] | Sense | 60 | TCAGCCTCTTCTCATTCCTGC |
Antisense | TTGGTGGTTTGCTACGACGTG | ||
rno-miR-26a [miRBase: MIMA0000796] | RT | - | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCTA |
Sense | 60 | CGCTTCAAGTAATCCAGGA | |
Antisense | GTGCAGGGTCCGAGGT | ||
U6 snRNA [GenBank:K00784] | Sense | 60 | CTCGCTTCGGCAGCACA |
Antisense | AACGCTTCACGAATTTGCGT |