Persistence and selection of an expanded B-cell clone in the setting of rituximab therapy for Sjögren’s syndrome

Table 1 CDR3 sequence features of expanded B-cell clones

Clone	VH	DH	JH	CDR3 (amino acid)
1-1	VH3-30-03*01	D3-3*01	JH6*03	CASPYYDFWSGYYMDYYYYYMDVW
1-2	VH3-30*03	DH6-19*01	JH5*01	CAKEAGSSGRAGWFDPW
2-1	VH1-69*01	DH4-2301, DH402	JH4*02	CARGTGDHTTVVTPFDYW
2-2	VH3-23*04	DH6-13*01	JH3*01	CAKAVAPVGSAYDVW
2-3	VH1-2*04	DH3-10*01	JH3*02	CARDSSGGGNDAFDMG
2-4	VH4-59*01	DH3-22*01	JH6*02	CARGMKVVAGYYYYGMDVW
2-5	VH3-23*04	DH6-19*01	JH3*02	CAKAAAVGSAYDIW
2-6	VH4-59*01	DH4-17*01	JH2*01	CAREDYGDYVRW
3-1	VH4-31*03	DH3-10*01	JH6*02	CAREGNTFIRGVIGWDPKPMDVW
3-2	VH1-46*01	DH3-10*01	JH4*02	CARDGSHYDFDYW
4-1	VH3-30*02	DH1-1*01	JH6*03	CARDSRGATGTSYYYYYMDVW
4-2	VH1-2*02	DH6-19*01	JH4*02	CARDAGSAGNYDTAVAGGGFVDYW

Shown are the best matches for the corresponding VH, DH, and JH gene alleles and the translated amino acid junction (CDR3) based upon the ImMunoGeneTics (IMGT) server output for all of the expanded B-cell clones. The clone numbers indicate the subject number followed by a unique identifier for the clone. For example, 2-1 refers to first (and largest) expanded clone in Sjögren’s syndrome subject 2 (SjS2). Expanded clones are molecularly defined as described in the text. DH, diversity gene segment; JH, joining gene segment; VH, variable gene segment.

ISSN: 1478-6362