|  | Reverse transcription PCR using |  | |||||
---|---|---|---|---|---|---|---|---|
 |  | Universal primers | Specific primers |  | ||||
Patient | Bacteria implicated | DNase | Non-DNase | Yer | Chlam | E. coli | Camp | Clinical details |
A | Yersinia | 6/20 | 2/20 | + | Â | + | Â | Acute ReA (see [10]); positiveve serology (agglutination 1:2500) and T cell responses |
B | Enteric | 10/20 | 7/20 | Â | Â | + | Â | Acute ReA following gastroenteritis; organism unknown |
C | Meningococci | Negative | 2/20 | Â | Â | + | Â | Acute postinfective arthritis; sore throat, rash, positive serology; daughter had meningococcal purpura and arthritis |
D | Streptococcus | 17/20 | 5/20 |  |  | + |  | Chronic erosive seronegative oligoarthritis; persistent positive serology – very high ASOT (>1200 U) and anti-Dnase |
E | Chlamydia | 5/20 | 5/20 | Â | + | + | Â | Acute sexually acquired ReA; high T-cell mediated responses to CT |
F | Yersinia (?) | 7/20 | 6/20 | Â | Â | + | Â | Acute (?) ReA; positive serology, B27+ and T-cell responses; no enteritis |
G | Unknown | 12/20 | 1/20 | Â | Â | + | Â | Chronic seronegative oligoarthritis; culture positive Campylobacter infection 2 years pre-arthritis |
H | Unknown | 11/20 | 5/20 | Â | Â | + | Â | Chronic seronegative oligoarthritis |
I | Yersinia | 8/20 | 9/20 | Â | Â | + | Â | Acute ReA following gastroenteriitis; positive serology (IgM 1:640, IgG 1:2560, IgA <1:80) and T-cell responses |
J | Chlamydia | 7/20 | 3/20 | Â | Â | + | Â | Acute sexually acquired ReA; high T-cell mediated responses to CT |
K | Campylobacter | 19/20 | 20/20 | Â | Â | + | Â | Psoriatic arthritis complicated by culture positive enteritis and flare in joint symptoms (?ReA) |
L | Campylobacter | 19/20 | 16/20 | Â | Â | + | Â | Acute ReA following enteric infection |
M | Campylobacter | Negative | Negative | Â | Â | Â | Â | Acute seronegative oligoarthritis and vasculitis; positiveve serology (IgM <1:80, IgG 1:640, IgA 1:160) |
N | Campylobacter | Negative | Negative | Â | Â | Â | Â | Sero-negative oligoarthritis; positive serology (IgM 1:640, IgG 1:640, IgA 1:160) and T-cell responses |