Figure 1
From: Heterogeneity of CD4 and CD8+ memory T cells in localized and generalized Wegener's granulomatosis
Representative flow-cytometric analysis of cell-surface chemokine receptor expression on CD4+ T cells. Peripheral blood mononuclear cells from a patient with localized Wegener's granulomatosis (WG), from a patient with generalized WG and from a healthy control were simultaneously stained with CD4–APC, CD45RO–PE, CD45–PerCP, and either CCR5-FITC or CCR3-FITC. CD4-positive lymphocytes were gated on the basis of light scattering properties and on CD4 and CD45 staining, then analyzed for expression of CD45RO and CCR5 or CCR3.