Figure 6
From: DEK binding to class II MHC Y-box sequences is gene- and allele-specific
DNase I footprinting localizes rDEK binding to the HLA-DQA1*0101 Y box. Partially purified rDEK (20–60 μl) was incubated with DQA1*0101 probe (antisense strand). Antisense rDEK (40 μl) (prepared simultaneously with the rDEK protein) was used as a control. The Y-box sequence is shown on the left. Arrows denote consistent protection against DNase I digestion in multiple assays. rAS-DEK = recombinant anti-sense DEK protein; rDEK = recombinant DEK protein.