Skip to main content

Table 2 Monocyte and PMN phenotypes

From: Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis

Phenotype

Patients with AAV

Healthy controls

P value

Monocytes (% of leukocytes)

4.4 ± 0.3

4.8 ± 0.24

n.s.

(109/L, reference range <1,1)

0.33 ± 0.02

  

Lymphocytes (% of leukocytes)

20 ± 1.2

31 ± 1.2

<0.0001

(109/L, reference range 1.1–4.8)

0.74 ± 0.05

  

PMN (% of leukocytes)

39 ± 1.3

28 ± 1.1

<0.0001

(109/L, reference range 1.7–8.0)

5.9 ± 0.3

  

CD16+CD10+ (% of PMN)

84 ± 1.1

82 ± 1.2

n.s.

CD16dimCD10- (% of PMN)

8.9 ± 0.8

6.8 ± 0.6

0.0109

CD177+ (% of PMN)

55 ± 2.4

47 ± 1.9

0.0075

CD10+CD16+ PMN

   

CD88+ (geoMFI)

442 ± 14

409 ± 15

n.s

CD62L+ (geoMFI)

1,277 ± 37

1,269 ± 41

n.s.

CD11c+(geoMFI)

558 ± 14

596 ± 16

n.s

CD10+CD16dim PMN

   

CD88+ (geoMFI)

391 ± 8.2

381 ± 7.6

n.s

CD62L+ (geoMFI)

450 ± 23

457 ± 22

n.s

CD11c+(geoMFI)

354 ± 7.2

428 ± 10

<0.0001

Monocytes

   

CD88+ (geoMFI)

162 ± 2.5

165 ± 2.5

n.s

CD62L+ (geoMFI)

770 ± 29

651 ± 37

<0.0001

CD11c+(geoMFI)

1,645 ± 63

2,079 ± 78

<0.0001

  1. The frequencies of monocytes, lymphocytes, polymorphonuclear leukocytes (PMN), CD10+CD16+ (mainly segment nucleated neutrophils) and CD10-CD16dim (suggested as a marker for newly released neutrophils) were investigated in healthy controls (n = 109) and patients (n = 105) using flow cytometry. In addition, the level of surface expression of CD88 (C5aR), CD62L and CD11c was studied on CD10+ CD16+ PMN, CD10 + CD16dim PMN and monocyte populations, as a measurement of activation and reported as geometric mean fluorescence intensity (geoMFI). The two-sided Mann-Whitney test was used to calculate the level of significance. Values are reported as mean ± SEM. AAV anti-neutrophil cytoplasmic antibodies associated vasculitides, n.s. not significant