Fig. 6

Elevated expression level of miR-142-3p affected moDCs-CD4⁺ T cells interaction. a Percentage of CD4⁺ T cells attracted by supernatants of moDCs in NC or empty lentiviral vector (VEC) or lentivirus (LV) group measured by a Transwell chamber. ^# P < 0.05 versus NC and VEC. b The percentage of CD4⁺CD25⁺Foxp3⁺ Tregs among CD4⁺ T cells co-cultured with moDCs was assessed using flow cytometry and data of one representative experiment (c) out of three independent experiments was shown. Foxp3⁺ cells were gated from CD4⁺CD25⁺ T cells. Data were shown as means ± SD of three independent experiments. ^# P < 0.05 versus NC and VEC. d and e The level of exocrine secretion of IL-17 (^# P < 0.001 versus NC and VEC) and IL-10 (^# P < 0.05 versus NC and VEC) in the supernatants derived from co-culture medium of CD4⁺T cells- moDCs. N = 3 in each group. Data were shown as means ± SD. IL interleukin