Fig. 2
Inhibition of Syk and PI3K reduces BCP crystal-induced IL-1 production in primary macrophages. Human macrophages (0.5 × 106 cells/well) were primed with LPS (100 ng/ml) for 2 h prior to treatment with a–c piceatannol (10 μM, 25 μM, and 50 μM) or d–f LY294002 (10 μM, 25 μM, and 50 μM) for 1 h and stimulation with BCP crystals (50 μg/ml) for 6 h. Cell supernatants were assessed for IL-1β, IL-1α, and TNF-α by ELISA. Results indicate mean (± SEM) of three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs LPS + BCP