Fig. 3

HMGB1 increased the binding of anti-MPO mAb to GEnCs. Compared with untreated cells, moesin expression and the binding of anti-MPO monoclonal antibody (mAb) were upregulated on HMGB1-treated GEnCs (a, b). Binding of anti-MPO mAb significantly increased on HMGB1-treated GEnCs compared with untreated cells (c). Bars represent mean ± SD of repeated measurements from three independent experiments. Representative histogram of effects of HMGB1 on the binding of anti-MPO monoclonal antibody to GEnCs (d). Proportion of GEnCs with both upregulated moesin expression and increased anti-MPO monoclonal antibody binding elevated in HMGB1-treated cells (e, f). Moesin on GEnCs could be captured by polyclonal antibodies to MPO by immunoprecipitation (g). HMGB1 high mobility group box-1, MPO myeloperoxidase, MFI mean fluorescence intensity