Fig. 1

EGR1 increased in the dermis of SSc patients and was located predominantly in fibroblasts. A–C co-staining of EGR1 with vimentin, CD31, or CD45 in skin tissues from healthy donors (n = 8) and SSc patients (n = 8). D Quantification of EGR-positive cells’ ratio in fibroblasts (VIMENTIN-positive), endothelia (CD31-positive), or leukocytes (CD45-positive). One-way ANOVA with Bonferroni multiple comparisons test was used for statistical analysis. Data are represented as mean ± standard deviation. *, 0.05 > P > 0.01; **, 0.01 > P > 0.001; ***, P < 0.001. E EGR1 expression level in skin cell clusters of a validation single-cell sequencing dataset. F EGR1 expression between diffused SSc (n = 12) and normal control (n = 10) from the validation single-cell sequencing dataset