Fig. 3

Iguratimod inhibited human skin fibroblast activation and function of extracellular matrix (ECM) synthesis. A Heatmap showed expression of collage, collage degradation system, and other major pro-fibrotic factors (p-value < 0.05). N = 3 per group. B mRNA expression of COL1A1, COL1A2, FN1, and ACTA2 in human dermal fibroblasts upon TGFβ stimulation and iguratimod treatment. N = 3 per group. *, 0.05 > P > 0.01; **, 0.01 > P > 0.001, one-way ANOVA with Bonferroni multiple comparisons test was used for statistical analysis. Data are represented as mean ± standard deviation. C Representative images of Sirius red for intracellular collagen detection (magnification: 40 ×) D Quantification of collagen content by eluting the Sirius red dye and colorimetric measuring. N = 3 per group. **, 0.01 > P > 0.001, one-way ANOVA with Bonferroni multiple comparisons test was used for statistical analysis. Data are represented as mean ± standard deviation. E Representative image of stress fiber via rhodamine-phalloidin staining along with quantification (magnification: 400 × , N = 4 per group), *, 0.05 > P > 0.01, **, 0.01 > P > 0.001, one-way ANOVA with Bonferroni multiple comparisons test was used for statistical analysis. Data are represented as mean ± standard deviation