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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: CircPTPN22 modulates T-cell activation by sponging miR-4689 to regulate S1PR1 expression in patients with systemic lupus erythematosus

Fig. 2

CircPTPN22 promoted Jurkat T-cell proliferation and cell cycle progression. A FISH assay to detect the subcellular localization of circPTPN22 in Jurkat T cells. The circPTPN22 probe was labelled with Cy-3. B, C Real-time PCR assay of circPTPN22 levels in Jurkat T cells transfected with LV-circPTPN22 or sh-circPTPN22. D, E CCK-8 assay to determine the proliferation of Jurkat T cells transfected with LV-circPTPN22 or sh-circPTPN22. F, G Flow cytometry analysis of apoptosis in Jurkat T cells transfected with LV-circPTPN22 or sh-circPTPN22. H, I Flow cytometry analysis of the proportion of cell cycle distribution in Jurkat T cells transfected with LV-circPTPN22 or sh-circPTPN22. The experiments were repeated three times. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Arthritis Research & Therapy

ISSN: 1478-6362

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