Fig. 4

S1PR1 is the target gene of miR-4689. A Venn diagram showing the common target genes of miR-4689 analysed using the TargetScan, miRDIP, miRDB, and miRWalk databases. B TargetScan indicated that miR-4689 had binding sites with S1PR1 in two positions. C Prediction of S1PR1 3'-UTR binding sites for miR-4689 using RNAhybrid tools. D The expression levels of S1PR1 mRNA in PBMCs from SLE patients and HCs were detected using real-time PCR. E Correlation analysis of miR-4689 and S1PR1 mRNA levels. F Correlation analysis of circPTPN22 and S1PR1 mRNA levels. G. ROC curve analysis of the sensitivity and specificity of S1PR1 in patients with SLE and HCs. H Western blotting results showing the protein expression level of S1PR1 in PBMCs from each group. I, J Expression levels of S1PR1 protein in Jurkat T cells transfected with miR-4689 mimics or inhibitor were determined by western blotting. K Binding sequence prediction of S1PR1-3' UTR-WT and miR-4689 and sequence construction of S1PR1-3' UTR-MUT. A luciferase reporter assay was used to validate the binding relationship between S1PR1 and miR-4689 in HEK-293 T cells. Mfe minimum free energy, SLE systemic lupus erythematosus, HCs healthy controls, ROC receiver operating characteristic, WT wild type, MUT mutant type. The experiments were repeated at least three times. *P < 0.05, ***P < 0.001, ****P < 0.0001; ns not significant