Fig. 2

RhoA regulates the type I IFN-stimulated response element (ISRE) and downstream gene expression. A ISRE activity of HEK293T cells transfected with ISRE-luciferase and a Renilla reporter plasmid together with a negative control (Ctrl) or RhoA-targeted siRNA (each at 200 nM). B ISRE activity of HEK293T cells transfected with RhoA-overexpression or control plasmids (each at 100 ng). At 24 h after transfection, the cells were left unstimulated (0 h) or stimulated with IFN-a (1000 U/mL, 6 h). Data in A and B are expressed as fold-change based on relative luciferase activities (ratio of firefly luciferase to Renilla luciferase). C–E The relative expression of OAS1, IFIT3, and CXCL10 mRNAs were determined by quantitative PCR. F The CXCL10 levels in culture supernatants were determined by enzyme-linked immunosorbent assay. Data were assessed in HEK293T (C, D) and THP1 cells (E, F) at 24 h after transfection of the negative control (Ctrl) or siRNA (200 nM) stimulated cells with IFN-a (1000 U/mL) for 6 h. Bars show the mean ± SEM of 3 independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 by Student’s t-test