Fig. 3

RhoA regulates type I IFN-induced STAT-1 phosphorylation. Western blot analysis (A, C) and densitometry histograms (B, D) of cell lysates of cultured HEK293T cells (5 × 10⁵ cells per well) transfected with siRNA (200 nM) targeting RhoA mRNA (A) or a RhoA expression plasmid vector (4 µg/mL) (C), and their controls (a negative control siRNA or pCMV-NC) stimulated with IFN-a (1000 U/mL) for different times, as shown. The staining density histograms are from three independent experiments and values are expressed as the ratios of phosphorylated STAT-1 protein to total STAT-1. The immunoblot of cells transfected with a negative control siRNA (B) or the pCMV-NC vectors (D) were set at a value of 1. *p < 0.05, **p < 0.01.by Student’s t-test