Fig. 5

The RhoA/ROCK inhibitor Y27632 reduces type I IFN signaling in lupus PBMCs. A Relative expression of OAS1 and CXCL10 mRNA in PBMCs obtained from SLE patients (n = 6) and incubated in medium alone or with Y27632 (60 μM, 6 h). B OAS1 and C. CXCL10 mRNA measurements in lysates of lupus PBMCs (n = 6) cultured with or without Y27632 (60 μM, 45 min) and stimulated with IFN-a (1000 U/mL). Results are the relative expression levels of OAS1 and CXCL10 mRNA normalized to endogenous GAPDH mRNA levels. D CXCL10 levels in culture supernatants quantified by ELISA. Bars show the mean ± SEM of 6 individual PBMC samples. *p < 0.05 by Student’s t-test