Table 1 Primer sequences
Rat gene | Primer sequences (5′–3′) | |
|---|---|---|
Aggrecan | Forward | GCAGCACAGACACTTCAGGA |
Reverse | CCCACTTTCTACAGGCAAGC | |
Col2a1 | Forward | GGCCAGGATGCCCGAAAATTA |
Reverse | ACCCCTCTCTCCCTTGTCAC | |
ADAMTS-5 | Forward | AGTACAGTTTGCCTACCGCC |
Reverse | GATTTGCCGTTAGGTGGGCA | |
MMP-3 | Forward | TTTGGCCGTCTCTTCCATCC |
Reverse | GCATCGATCTTCTGGACGGT | |
TNF-α | Forward | CATCCGTTCTCTACCCAGCC |
Reverse | AATTCTGAGCCCGGAGTTGG | |
IL-1β | Forward | AGGCTGACAGACCCCAAAAG |
Reverse | CTCCACGGGCAAGACATAGG | |
CGRP | Forward | CAGTGAAGAAGAAGCTCGCCTA |
Reverse | CAGTGTTGCAGGATCTCTTCTG | |
β-Actin | Forward | CTATGAGGGTTACGCGCTCC |
Reverse | ATGTCACGCACGATTTCCCT | |
P. acnes 16S rDNA | Forward | GGGTTGTAAACCGCTTTCGCCT |
Reverse | GGCACACCCATCTCTGAGCAC | |
S. aureus 16S rDNA | Forward | AGAGTTTGATYMTGGCTCAG |
Reverse | ACGGYTACCTTGTTACGACCT | |