Fig. 2

Clonal trajectories of memory B cell differentiation in pSS SGs. A Network visualization of all IgH CDR3 amino acid sequences from scBCRseq. Sequences separated by Levenshtein distances < 4 were connected by edges. Each node reflects a single B cell; nodes colored by tissue origin. Four hundred fifteen SG nodes (of 1446 SG cells) and 265 PB nodes (of 2032 PB cells) with at least one connection were retained in the network. B Bar plot breakdown of the classification of each edge. Mixed edges connect PB and SG nodes. C Network visualization as in A with cell types annotated using node color. D Bar plot breakdown of each edge according to the connected cell types. E UMAP visualization of the inferred trajectory for naïve-to-memory B cell differentiation within labial gland B cells. Arrowed curve indicates direction. F–G Distribution of the correlation of each Reactome pathway calculated using TIPS with overall pseudotime progression (F). Eight pathways with correlation > 0.5 are shown in G