Fig. 3

Building a database of observed BCRs in pSS. A–C Histogram mapping the count distribution of each observed gland-infiltrating B cell BCR, segregated by chain. D Network visualization of clustering for 5000 randomly downsampled CDR3 AA sequences taken from controls. Nodes with LV distance < 4 are connected by edges. E Clustering of 5000 randomly downsampled sequences, as in D, of sequences from pSS patients. F Summary of connected node count in the control and pSS groups (5 random downsamples). Student’s two-tailed t-test p = 1.89 × 10⁻⁶. G Summary of the average clustering coefficient of each network, calculated in Gephi. Student’s two-tailed t-test p = 8.17 × 10⁻³