Fig. 8

Knockdown of circFTO ameliorates RA in vivo. (A) The expression of circFTO in synovial tissues was quantified using qRT-PCR. (B) Clinical scores of CIA model mice were assessed in each experimental group. (C) Representative images depicting the severity of RA in CIA-induced mice were captured. 4. (D) Representative images and semi-quantitative analysis of hematoxylin and eosin staining were performed on cartilage tissues from CIA-induced mice. (E-G) The protein expression of WTAP, METTL14, METTL3, SOX9, proliferative markers (Ki67 and PCNA), and apoptotic markers (BAX, cleaved-caspase-3, and cleaved-caspase-9) in articular cartilage were detected by western blotting. (H) The mRNA expression of metabolic markers (MMP-13, ADAMTS-5, COL2 and Aggrecan) in articular cartilage was detected by qRT-PCR. Data are presented as mean ± SD, n = 6; *p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.001, #p < 0.05, ##p < 0.01