Fig. 5
From: LATS2 degradation promoted fibrosis damage and rescued by vitamin K3 in lupus nephritis
LATS2 was downregulated in K48 ubiquitin–proteasome way. A, Overexpression of LATS2 regulated CTGF production, and YAP downstream genes. B, TGF-β promoted LATS2 ubiquitination in HK-2 cells. C, K48R mutation of Ub protected LATS2 ubiquitinated markers in HK-2 cells. D, KEGG pathway analysis of interacted proteins with LATS2. E, PSMs of LATS2 and SIAH2 in IP-MS analysis. F, TGF-β treatment increased SIAH2 and promoted YAP activation in HK-2 cells. G, Expression of SIAH2 and Zyxin in LN mice by IHC staining (scale bar, 20 μm). H, Western blot analysis of SIAH2-LATS2 axis in LN mice. I, Dual-luciferase analysis of the interaction between RUNX1 and SIAH2-promoter, RUNX1 promoted luciferase transcription in pGL3-SIAH2p. J, Ch-IP qPCR analysis of the SIAH2 transcription regulated by RUNX1