Fig. 6

RELA KO chondrocyte pellets display ECM deposition compared to WT cells, and they are more resistant to IL-1β induced catabolism. Infant articular cartilage was used as a A positive and B negative histological control. Safranin O, collagen type I and II immunostaining of C polydactyly chondrocytes, D FE002 chondroprogenitor cells, and E OA chondrocyte pellets in an untreated condition after 21 days of culture in chondrogenic media. Safranin O, collagen type I and II immunostaining of F polydactyly chondrocytes, G FE002 chondroprogenitor cells, and H OA chondrocytes treated with 10 ng/ml of IL-1β in the last week of culture in chondrogenic media. I-K Quantification of the relative staining intensity. 20X pictures scale bar, 100 µm; low magnification 2X scale bar, 500 µm. One donor per cell type is showed as a representative example