Fig. 4
From: TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis
The effect of STING on chondrocytes TfR1 expression and iron influx. (A-B) Chondrocytes were treated with 5ng/ml IL-1β for 12 h with or without STING siRNA, then expressions of p-P65, P65, IL-6 and IL-1β were examined by western blotting. GAPDH was included as a loading control and semi-quantitative analysis of band density was conducted. (C-D) Chondrocytes were treated with 5ng/ml IL-1β with or without STING siRNA, representative images for ferrous ions in the indicated group and statistical analysis of fluorescence intensity. Scale bars = 200 μm. (E) Chondrocytes were treated with 5ng/ml IL-1β for 12 h with or without STING siRNA, then expressions of TfR1 were examined by western blotting. GAPDH was included as a loading control and semi-quantitative analysis of band density was conducted. (F) Representative images of IF staining for TfR1 expression in chondrocytes after STING inhibition. Scale bars = 50 μm. Data are presented as mean ± SD from three independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001