Fig. 5
From: TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis
The effect of TfR1 on HIF-1α/BNIP3 mediated mitophagy process. (A) Representative western blotting images of HIF-1α, BNIP3 and semi-quantitative analysis of band density in IL-1β treated chondrocytes with or without TfR1 siRNA. (B) Representative immunofluorescence images of BNIP3. Mitochondria were stained with red mitotracker probe, BNIP3 were stained with green immunofluorescence. (C) Representative western blotting images of Drp-1, MFF and semi-quantitative analysis of band density in IL-1β treated chondrocytes with or without TfR1 siRNA. (D) Representative immunofluorescence images of Drp1. Mitochondria were stained with red mitotracker probe, BNIP3 were stained with green immunofluorescence. (E-F) Western blot analysis of SOX9, COL2, MMP13, MMP3, iNOS and COX2 expression in chondrocytes transfected with TfR1 siRNA with or without 3-MA and semi-quantitative analysis. Data are presented as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001