Fig. 6
From: TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis
Fer-II inhibited TfR1 expression and inhibited cartilage ECM degradation. (A-B) Chondrocytes were treated with 5ng/ml IL-1β with or without TfR1 siRNA, representative images for ferrous ions in the indicated group and statistical analysis of fluorescence intensity. Scale bars = 200 μm. (C-D) Chondrocytes were treated with 5ng/ml IL-1β for 12 h with or without Fer-II, then expressions of TfR1, MMP3, MMP13, SOX9 and COL2 were examined by western blotting. GAPDH was included as a loading control and semi-quantitative analysis of band density was conducted. (E-F) Representative images of IF staining for COL2 and GPX4 expression in chondrocytes treated with IL-1β for 12 h with or without Fer-II. Scale bars = 50 μm. (G-J) Representative western blotting images of GPX4, SLC7A11, FTH, c-GAS, STING and semi-quantitative analysis of band density in IL-1β treated chondrocytes with or without Fer-II. Data are presented as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001