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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Nintedanib downregulates the profibrotic M2 phenotype in cultured monocyte-derived macrophages obtained from systemic sclerosis patients affected by interstitial lung disease

Fig. 2

Western blotting with related densitometric analysis of the protein synthesis and gene expression of surface and functional markers of cultured MDMs from HS, SSc-ILD patients and SSc patients without ILD. (A) Evaluation by Western blotting and related densitometric analysis of the protein synthesis of CD204, CD206, CD163, and MerTK, and (B) evaluation by quantitative real time polymerase chain reaction (qRT-PCR) of the gene expression of MerTK and TGFβ1 in cultures of monocyte-derived macrophages (MDMs) obtained from 5 voluntary healthy subjects (HS), 4 SSc patients without ILD (SSc patients no-ILD, SSc pts no-ILD), and 10 SSc-ILD patients (SSc-ILD pts). Cultured MDMs were maintained in normal growth medium without any treatment for 24 h. The value of protein expression of CD204, CD206, CD163, and MerTK was normalized to that of the corresponding GAPDH in cultured HS-MDMs, MDMs from SSc pts no-ILD, and MDMs from SSc-ILD pts. The resulting value of the protein expression of each molecule in cultured MDMs from SSc-ILD pts and from SSc pts no-ILD was compared with that obtained in cultured HS-MDMs (taken as unit value). Gene expression of MerTK and TGFβ1 corresponds to the expression level (fold-increase) of the target gene in cultured MDMs from SSc pts no-ILD, and MDMs from SSc-ILD pts was compared with that obtained in cultured HS-MDMs (taken as unit value). Data are reported as median with range. The protein and gene expression of each molecule obtained in cultured MDMs from SSc pts no-ILD and SSc-ILD represent the fold increase compared to the unit value of cultured HS-MDMs. Data are reported as median with range of fold increase compared to HSs

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Arthritis Research & Therapy

ISSN: 1478-6362

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