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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Nintedanib downregulates the profibrotic M2 phenotype in cultured monocyte-derived macrophages obtained from systemic sclerosis patients affected by interstitial lung disease

Fig. 3

Gene expression of cell membrane and functional markers of profibrotic M2 phenotype in cultured MDMs obtained from SSc-ILD patients. Evaluation by quantitative real time polymerase chain reaction (qRT-PCR) of the gene expression of CD204, CD206, CD163, MerTK, IL10, and TGFβ1 in cultures of monocyte-derived macrophages (MDMs) obtained from 10 SSc-ILD patients. Cultured MDMs were maintained in normal growth medium without any treatment (black bar), treated with nintedanib at the concentration of 0.1µM (light gray bar), treated with nintedanib at the concentration of 1µM (dark gray bar) for 3, 16, and 24 h. Gene expression corresponds to the expression level (fold-increase) of the target gene of nintedanib-treated SSc macrophages compared with that of untreated cells, taken as the unit value. Data are reported as median with range of fold increase compared to untreated cells

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Arthritis Research & Therapy

ISSN: 1478-6362

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