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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Nintedanib downregulates the profibrotic M2 phenotype in cultured monocyte-derived macrophages obtained from systemic sclerosis patients affected by interstitial lung disease

Fig. 4

Western blotting and related densitometric analysis of the protein synthesis of surface and functional markers of cultured MDMs from SSc-ILD patients. Evaluation by Western blotting and related densitometric analysis of the protein synthesis of CD204, CD206, CD163, and MerTK in cultured monocyte-derived macrophages (MDMs) obtained from 10 SSc-ILD patients. Cultured MDMs were maintained in normal growth medium without any treatment (untreated cells, black bar), treated with nintedanib at the concentration of 0.1µM (light gray bar), treated with nintedanib at the concentration of 1µM (dark gray bar) for 3, 16, and 24 h. For each experimental condition, the value of protein synthesis of CD204, CD206, CD163, and MerTK was normalized to that of the corresponding GAPDH. The resulting value of each treatment was compared with that of the related untreated cells (taken as unit value). Data are reported as median with range of fold increase compared to untreated cells

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Arthritis Research & Therapy

ISSN: 1478-6362

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