- Paper Report
- Open Access
Macrophage derived cytokine and nuclear factor ?B in patients with psoriatic arthritis
- Oliver Fitzgerald1
© Current Science Ltd 2000
- Published: 19 July 2000
- Monocyte derived cytokines
- psoriatic arthritis
- synovial memebrane
The expression of monocyte-derived cytokines and the transcription factor nuclear factor-?B (NF-?B) has not previously been analysed in SM or skin samples derived from patients with PsA. The elucidation of the cytokine pathways involved in PsA may have therapeutic implications. To analyse the expression of monocyte-derived cytokines in SM and skin of patients with PsA compared to SM from patients with rheumatoid arthritis (RA).
While there was no difference in sublining cellular infiltration, lining layer thickness and CD68+ macrophage infiltration were reduced in PsA patients. SM staining for tumour necrosis factor-a, interleukin (IL)-1a, IL-1?, IL-15 and IL-10 was predominately localised to the lining layer and perivascular macrophages. In general, expression of these cytokines was reduced in PsA patients as compared to RA patients and this was thought likely to relate to the lower macrophage numbers. No data on serial sections or from double staining, however, were presented. In keeping with reduced cytokine expression, the expression of NF-?B was lower in PsA than in RA sublining. Finally, while a similar pattern of cytokine staining was noted in the skin, perilesional dermis was noted to have higher levels of IL-15 and increased marked basal keratinocyte staining of IL-10 was observed in perilesional as compared to lesional skin.
Blind-needle biopsy samples were obtained from 34 patients with PsA and from 7 patients with RA. An additional 13 synovial tissue samples were obtained at the time of surgical synovectomy from patients with RA and 5 SM samples were obtained from osteoarthritis patients at arthroplasty. In 25 PsA patients, skin biopsies were also obtained. More than one third of the SM samples obtained from PsA patients were considered non-evaluable. Standard immunohistochemical techniques were used to quantify the cellular infiltrate and the cytokine and NF-?B localisation.