- Paper Report
- Open Access
CD4+CD25+regulatory T cells directly act on antigen-presenting cells by downregulation of CD80/CD86 costimulatory molecules
- Fanny Monneaux1
© Current Science Ltd 2000
- Published: 22 August 2000
- CD4+ CD25+
- regulatory T cell
Elimination of CD4+CD25+ T cells, which constitute 5-10% of peripheral CD4+ T cells in normal naive mice, leads to spontaneous development of various autoimmune diseases. When stimulated, these regulatory T cells suppress the proliferation of the CD4+CD25- T cells, resulting in reduced IL-2 production by CD4+CD25- T cells. Suppression depends on cellular interactions with APCs. The effector functions of regulatory T cells still remain unclear. Identifying the molecules involved in the interaction between regulatory cells and the naive T cells would contribute to our understanding of the causes and mechanisms involved in autoimmune diseases. To further elucidate the mechanism of CD4+CD25+ T cell suppression on CD4+CD25- T cells.
CD4+CD25+ T cells suppress responses of CD4+CD25- T cells stimulated with anti-CD3 antibody and cultured with either B cells (100% suppression with 1:4 ratio of CD25+/CD25-) or DCs (100% suppression with 9:1 ratio of CD25+/CD25-). The expression of costimulatory molecules (both CD80 and CD86) on DCs was decreased when graded numbers of CD4+CD25+ T cells were added to the coculture, even if DCs were first stimulated with anti-CD40 antibody. Surprisingly, CD4+CD25+ cells downregulate CD80 mRNA levels on DCs, whereas levels of CD86 mRNA were not significantly decreased. This suggests that the expression of these two molecules is regulated by different mechanisms. The down regulation of CD80 and CD86 molecules on DCs depends on direct interaction between CD4+CD25+ T cells and DC interaction, and no decreased expression was observed when DC were separated from CD4+CD25+ cells by a cell permeable membrane.
CD4+ T cell populations were recovered by negative selection, and CD4+CD25+ and CD4+CD25- subpopulations were then separated by magnetic cell sorting (MACS technology). Splenic B cells and DCs were enriched with B220-antibody-congugated beads and CD11c conjugated beads, respectively. Suppressor cell activity was assessed by coculturing various numbers of CD4+CD25+ T cells with CD4+CD25- T cells and various numbers of B cells or DCs in 96-well plates, and stimulating with anti-CD3 antibodies. Proliferation was measured after 3 days of culture. For flow cytometric analysis, cells were cocultured 48 h in 24-well plates. mRNA levels for CD80 and CD86 were determined by RT-PCR with specific primers for CD80, CD86, and hypoxanthine guanine phosphoribosyltransferase (HPRT) as internal control.