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- Open Access
Can two different TCRs that react with the same peptide-MHC complex influence the differentiation of naive CD4 T cells?
- Petya Dimitrova1
© Current Science Ltd 2000
- Published: 22 August 2000
- T cell
- transgenic mice
Several studies have demonstrated that antigenic peptides can modulate the magnitude of T cell activation by influencing the rate of phosphorylation by protein kinases in the T cells and by controlling the direction of the functional immune response. For example, high doses of moth cytochrome c peptide induced a Th1 commitment in vivo, while low doses of the peptide drove Th2 differentiation. Likewise, agonist peptides forming high affinity interactions with the MHC molecule favored Th1 differentiation of naive CD4+ T cells, whereas altered peptide ligands with affinities either for the presenting MHC or for the TCR favored Th2 differentiation. Moreover, the MHC haplotype itself might influence T cell differentiation as MHC molecules that bound to a peptide with high avidity promoted Th1 differentiation, in contrast to MHC molecules expressing a lower avidity for the given peptide, which favored Th2 differentiation. To investigate whether a single amino acid alteration in the TCR's peptide contacting residues affects the differentiation of naive CD4+T cells in response to priming with antigenic peptide.
Alteration of the TCR a chain at position 51 led to a striking reduction in the number of CD4+ T cells that expressed the transgenic TCR in secondary lymphoid tissue and resulted in lower proliferative responses to both peptides, with a more prominent effect on the response to the wild-type peptide CA-WT. Interestingly, after priming with CA-WT peptide 33% of sorted L51S naive CD4+ T cells became IL-4-producers compared to 7% in D10.BR T cells. In contrast, 12% of the L51S cells secreted IFN-? after priming with CA-WT compared to 28% in D10.BR T cells. Different doses of CA-WT did not result in significant IFN-? production by L51S CD4+ T cells. In contrast, high doses of both peptides, R2G and CA-WT, promoted Th1 differentiation of D10.BR CD4+T cells, whereas low doses favored Th2 commitment.
CD4+ T cells from TCR transgenic mice, expressing either a wild-type TCR specific for chicken conalbumin (D10.BR mice) or an altered TCR bearing a leucine-to-serine substitution at position 51 in CDR2 of the TCR a chain (L51S mice), were isolated from lymph nodes and spleen. Function and differentiation of naive CD4+ T cells into Th1 or Th2 effectors was assessed after primary and secondary stimulation with two peptides from chicken conalbumin, the wild type (CA-WT) and a mutated peptide, R2G.
- Blander JM, Sant'Angelo DB, Bottomly K, Janeway CA: Alteration at a single amino acid residue in the T cell receptor a chain complementarity determining region 2 changes the differentiation of naive CD4 T cells in response to antigen from T helper cell type 1 (Th1) to Th2. J Exp Med. 2000, 191: 2065-2073.PubMedPubMed CentralView ArticleGoogle Scholar