- Paper Report
- Open Access
Reconstitution of the lymphoid compartment by naive T cells
- Andrew Cope1
© Current Science Ltd 2000
- Published: 19 September 2000
- T cells
CD8+ or CD4+ T lymphocytes die in the absence of MHC class I or class II molecules. This indicates that the interaction between the T cell receptor (TCR) and self peptide/MHC molecules delivers survival signals in the peripheral T cell compartment. During the recovery from profound lymphopenia in vivo, peripheral T cell numbers recover rapidly and acquire a memory T cell phenotype, a process termed homeostatic proliferation. Until now, this process was thought to involve the reconstitution of the T cell pool by memory T cells. Using a TCR transgenic model, the Bevan laboratory explored whether this process might involve the expansion of naive CD8+ T cells.
Following adoptive transfer of naive TCR transgenic CD8+ T cells into syngeneic irradiated recipients, >80% of donor T cells proliferate and express a memory-like phenotype, acquiring CD44, Ly6 and interleukin-2RÎ±, but not CD25, CD49d or CD69. Nor do they downregulate CD62L or the TCR. The proliferation rate stabilises once the lymphoid compartment approaches normal cellularity, with T cells reverting to the naive phenotype. Despite this expansion, T cells do not exhibit CTL effector function in the absence of exogenous antigen; however, transfer into RAG1(-/-) hosts leads to sustained proliferation and CTL activity.
Adoptive transfer, TCR transgenic mice, RAG-1 deficient mice