- Paper Report
- Open Access
Markers of tumor endothelium-Implications for anti-angiogenic therapies
- Oliver Distler1
© Current Science Ltd 2000
- Published: 13 October 2000
- endothelial cells
- differential gene expression
Tumors require a sufficient blood supply for their expansive growth. The aim of this study was to identify tumor-specific endothelial markers that might be candidates for a specific anti-angiogenic tumor therapy.
In total 79 genes were identified that were differentially expressed between endothelial cells derived from tumor endothelium and normal colonic mucosa. The expression of 33 of these genes was elevated at least 10-fold in tumor endothelial cells, including 11 known and 14 so-far uncharacterized genes. In situ hybridization on tissue samples confirmed that the expression of eight of the nine uncharacterized genes that were studied in depth were specific for tumor endothelial cells. Moreover, these genes were also expressed on endothelial cells of other tumors including lung and brain tumors. Except for one gene, these genes were also expressed at elevated levels in other angiogenic states such as healing wounds.
Magnetic beads, RT-PCR, serial analysis of gene expression (SAGE), in situ hybridization, immunochemistry