- Paper Report
- Open Access
IFN maintains anergic cell survival
- Katherine Wilson1
© Current Science Ltd 2000
- Published: 13 October 2000
Following suboptimal stimulation through the T cell receptor, cells either undergo apoptosis or become anergic. Anergic cells have been shown to survive for extended periods in vivo, suggesting that anergic cells must be able to escape apoptosis. Previous studies have demonstrated that interferan (IFN)-Î²Î± can prevent T cell apoptosis induced by cytokine deprivation. This study aimed to show that IFN-Î²Î± is the major survival factor for anergic T cells.
T cell clones were incubated with specific peptide in the absence of antigen-presenting cells. This T:T presentation led to cell death mediated by CD95. When cells were co-cultured with fibroblasts or fibroblast-conditioned media, cell death was significantly reduced. Addition of anti-IFN-Î± antibodies to the media significantly reduced the anti-apoptotic activity. IFN-Î± prevented apoptosis by inducing STAT-1 activity and blocking protein kinase C-Î´ translocation to the nucleus. Importantly this study demonstrated that the cells rescued from cell death were all anergic. The authors conclude that IFN-Î²Î± rescues T cells from CD95-mediated apoptosis; this mechanism of rescue may account for the persistence of anergic cells in vivo.
Co-culture, anergy induction, STAT analysis, PKC staining