- Paper Report
- Open Access
H-chain CDR3 contribution in specificity of autoantibodies
- Fanny Monneaux1
© Biomed Central Ltd 2001
- Published: 12 January 2001
- complementarity-determining region
Systemic lupus erythematosus is characterized by the production of antibodies directed against various nuclear antigens. Many groups have focused on the sequence of the V region genes of antinuclear antibodies and several features accounting for DNA binding have emerged from these sequences, especially in VH-CDR (H3). In this study, the authors exchanged H3 domains between the 3H9 antibody and two donor antibodies, LG8-1 (closely related to 3H9) and ASWA1 (divergent from 3H9). The H3 domain of these three antibodies differed in the number of arginine residues and in overall charge and these antibodies share different specificities. Variant H chains were expressed together with the 3H9 light chain as single-chain Fv (scFv), and the five antibodies were then tested for their ability to bind nucleosomes, DNA and cardiolipin.
The five scFvs equally bind cardiolipin, suggesting that this recognition is not due to junctional or somatic diversity mechanisms. In contrast, the DNA and nucleosome binding activity was only observed for 3H9 scFv with its own H3. It is surprising that the exchange with LG8-1 H3, which is arginine-rich, is not sufficient to restore the ability of this antibody to bind DNA. These results suggest that conformation, flexibility or additional sidechain contributions of 3H9 H3 are crucial, and are directly involved in DNA and nucleosome binding.
scFv construction and expression, ELISA, DNA sequencing